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Table 2 DAVID functional annotation clusters for all proteins identified in oEVs

From: Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo

Most descriptive categories of DAVID functional annotation clusters groups by similar GO terms # Proteins1 Score2
GO Group 1: Vesicle/exosome/secretion 271 114.5
extracellular exosome (263, 4.2)3, vesicle membrane (32, 2.8), exocytosis (30, 3.9), secretion (50, 2.4)   
GO Group 2: Cell adhesion/junction 141 49.9
adherens junction (106, 6.9), cell-cell junction (61, 4.3) cell-cell adhesion (71, 3.2), cell junction organization (13, 2.7)   
GO Group 3: Protein secretion/folding 129 29.1
protein targeting to ER (42, 21.1), protein folding (27, 6.2), Protein processing in endoplasmic reticulum (15, 2.8), regulation of secretion (23, 1.8)   
GO Group 4: Gene expression 168 21.1
poly(A) RNA binding (102, 4.3), gene expression (137, 1.4), RNA biosynthetic process (87, 1.2)   
GO Group 5: Wound healing/coagulation 154 9.9
regulation of body fluid levels (38, 4), coagulation (31, 4.5), wound healing (37, 3.6), platelet activation (21, 6.8), blood circulation (19, 5.8)   
GO Group 6: Small molecule binding 109 10.6
nucleoside-triphosphatase activity (47, 3), small molecule binding (96, 1.9), ATP binding (57, 1.9), GTPase activity (22, 4.7)   
GO Group 7: Signal transduction 137 7
posttranscriptional regulation of gene expression (38, 4,2), regulation of protein modification process (62, 2), protein phosphorylation (59, 1.7), regulation of cell communication (85, 1.5)   
GO Group 8: Proteolysis/cell cycle 89 6
proteasome complex (14, 9.6), mitotic cell cycle (37, 3.2), proteolysis (57, 1.7), endopeptidase activity (21, 2.1)   
GO Group 9: Cytoskeleton organization 95 5.4
cytoskeleton organization (45, 2.1), microtubule cytoskeleton (51, 2.1), contractile fiber (15, 3), actin filament organization (19, 3), Ras GTPase binding (13, 2.4)   
GO Group 10: Apoptosis 66 5.2
programmed cell death (61, 1.7), intrinsic apoptotic signaling pathway (12, 2.3), negative regulation of intracellular signal transduction (16, 1.8)   
GO Group 11: Protein stabilization/sperm-zona pellucida interaction 21 4.9
protein stabilization (18, 6.9), zona pellucida receptor complex (6, 30.1), binding of sperm to zona pellucida (7, 10.2), fertilization (10, 3.2)   
GO Group 12: Carbohydrate derivative metabolism 60 3.8
carboxylic acid metabolic process (35, 2.2), NADH metabolic process (9, 12.7), nucleotide metabolic process (29, 2.3), carbohydrate metabolic process (25, 1.7)   
GO Group 13: Protein biosynthesis 19 3.7
Protein biosynthesis (16, 6.7), tRNA aminoacylation (8, 7.9), translational elongation (9, 3.5)   
GO Group 14: Cell motility 52 3.4
locomotion (52, 1.8), cell motility (47, 1.9), cell migration (42, 1.9)   
GO Group 15: Oxidative stress 24 3.4
response to oxidative stress (20, 2.7), reactive oxygen species metabolic process (14, 3.3)   
GO Group 16: Cell development 62 3.1
cell development (51, 1.4), epithelial cell differentiation (20, 1.9), establishment or maintenance of cell polarity (12, 3.7)   
GO Group 17: Ion homeostasis 67 2.2
iron ion homeostasis (6, 4.4), homeostatic process (56, 1.8), ion homeostasis (22, 1.6), regulation of ion transport (21, 2.0)   
GO Group 18: Others 30 7.9
Annexin (9, 40.7), calcium-dependent protein binding (13, 11.2), phospholipid binding (19, 2.7), high-density lipoprotein particle (4, 6.9), steroid binding (6, 3.4)   
  1. Table shows DAVID functional annotation clusters with a score ≥ 1.3. 1number of unique proteins in each GO group;2highest enrichment score (geometric mean of member’s p-values of the corresponding annotation cluster in -log10 scale) of each group; 3in brackets: number of genes and fold enrichment for the functional term