Fig. 1

Iron-independent vs. iron-dependent essential genes in S. Typhimurium. Essential genes of S. Typhimurium were identified using Tn-seq method in LB medum (iron-replete) and LB medium supplemented with 2,2`-Dipyridyl (Dip; iron-restricted). Among all 336 essential genes identified, 215 genes were essential under both conditions (iron-independent essential genes), while 121 genes were essential only under iron-replete condition (iron-dependent essential genes). The iron-dependent essential genes included 11 genes encoding well known antibiotic targets. The read counts from LB-III (LB) and Dip400 (Dip) were used to calculate mutant fitness as expressed in Log₂ fold change