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Fig. 5 | BMC Genomics

Fig. 5

From: Unsupervised correction of gene-independent cell responses to CRISPR-Cas9 targeting

Fig. 5

CRISPRcleanR corrected sgRNA counts and downstream analysis with MAGeCK. a and b Normalised counts of sgRNAs of the transfected libraries versus the control plasmid for FE and non-essential genes (first two rows of plots), CN amplified genes (third row) and CN non-expressed genes (fourth row), for two example cell lines before (first and third column) and after (second and fourth column) CRISPRcleanR correction. Essentialities for CN-amplified cancer driver genes such as MYC, ERBB2 and CCND1 are retained post correction. For the sake of readability only genes with at least 10 copies have been highlighted. c Comparison of recall using MAGeCK for sgRNAs targeting genes in six predefined gene sets when using as input CRISPRcleanR uncorrected and corrected sgRNAs counts

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