Fig. 2

Cytological analysis of replication and dynamics of transcription during larval fat body development. Representative immunofluorescence images of 2nd-instar (a-d), mid-3rd instar (e-h), and late-3rd instar (i-l) larval fat body. ORC2 staining (a, e, i) is shown as green in the merged images. EdU staining (b, f, j) is depicted as red, and DAPI (c, g, k) is depicted in blue. Scale bar represents 100 μm. m,n Expression of transcripts within UR regions. m Expression profile heatmap of the 146 transcripts within the 7 UR regions common to all five tissues profiled by aCGH, including the adult midgut (MG) and adult Malpighian tubules (MpT) (late-3rd-instar larva data from [15]). n Fraction of expressed transcripts within the 7 common UR regions and fully replicated regions for the indicated tissues. Only transcripts with FPKM values of ≥3 are considered expressed