Fig. 4

Analysis of ORC binding in the larval fat body (FB). a aCGH profile of late-3rd-instar larval fat body (data from [15]) and the peak summit locations of ORC2 in the 2nd-instar, mid-3rd-instar, and late-3rd-instar larval fat body on chr2L with dm3 genome coordinates shown. UR regions in the late-3rd-instar larval fat body are denoted as black boxes and are highlighted by green shading. Regions that are underreplicated in other larval tissues but are fully replicated in late-3rd-instar larval fat body are denoted as red boxes. b Comparison of the number of fat body ORC2 binding sites per 100 kb within the late-3rd-instar larval fat body UR regions relative to fully replicated regions at different larval stages. * p < 10^− 5. c Comparison of the number of fat body ORC2 binding sites per 100 kb within regions that are underreplicated in other larval tissues but are fully replicated in late-3rd-instar larval fat body relative to fully replicated regions at different larval stages. *p < 0.001. For the analyses in panels B and C, to be conservative all ORC2 binding sites were considered, not solely the high confidence set