Fig. 6

Growth of plasmid-cured variants of L. lactis FM03P in LM17. a Growth curves of the plasmid-cured variants. Dotted lines represent the standard deviation of representative biological duplicates. The dashed line represents an OD600 of 0.2, which was used as threshold to determine the time to detection. b Maximum growth rate as function of the total plasmid size calculated using the two-fold dilution method. Numbers near the symbols correspond to the different plasmid-cured variants. The maximum growth rate of the parent strain FM03P could not be estimated using the two-fold dilution method due to flocculation affecting the optical density measurement and thus the time to detection and is therefore omitted. Error bars represent the standard deviation of biological triplicates