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Table 2 Overview of the putative genes and their putative functions

From: Large plasmidome of dairy Lactococcus lactis subsp. lactis biovar diacetylactis FM03P encodes technological functions and appears highly unstable

Plasmid

Gene

Putative function

Best homolog found in:

pLd1

repB

Replication initiator protein

 

orf2*

Replication-associated protein

 

hsdS

Type I restriction-modification system specificity subunit S

 

orf4

Hypothetical protein

 

orf5

Hypothetical protein

 

orf6

Hypothetical protein

 

tra

Transposase IS982 family

 

citQ

Leader peptide CitQ

 

citR

Translational regulator

 

citP

Citrate transporter

 

orf11

Hypothetical protein

 

pLd2

repB

Replication initiator protein

 

orf2*

Replication-associated protein

 

hsdS

Type I restriction-modification system specificity subunit S

 

cadA

Cadmium-transporting ATPase

 

cadC

Cadmium efflux system accessory protein

 

cspC

Cold shock protein

 

orf7

Serine/threonine protein phosphatase

 

tra

Transposase IS6 family

 

mobC

Mobilisation protein

 

mobA

Mobilisation protein

 

mobB

Mobilisation protein

 

mobD

Mobilisation protein

 

orf13

HXXEE domain-containing protein

Streptococcus thermophilus

orf14*

XRE family transcriptional regulator

Streptococcus thermophilus

repC*

Replication initiator protein

Lactobacillus farciminis

orf16

Hypothetical protein

Enterococcus faecium

pLd3

repC

Replication initiator protein

 

orf2

Hypothetical protein

Lactobacillus reuteri

pLd4

repB

Replication initiator protein

 

orf2

Hypothetical protein

Enterococcus faecalis

orf3

Hypothetical protein

Enterococcus faecalis

orf4

Hypothetical protein

 

orf5

Hypothetical protein

 

orf6

Hypothetical protein

 

fmtB

Peptidoglycan-binding protein

Streptococcus thermophilus

tra

Transposase IS982 family

 

orf9*

DNA-directed DNA polymerase

 

orf10

Hypothetical protein

 

tnpR

Resolvase

 

pLd5

repB

Replication initiator protein

 

orf2

Replication-associated protein

 

hsdS

Type I restriction-modification system specificity subunit S

 

tra

Transposase IS982 family

 

orf5

Hypothetical protein

 

orf6

Site-specific integrase

 

pLd6

repB

Replication initiator protein

 

orf2*

Hypothetical protein

 

hsdS

Type I restriction-modification system specificity subunit S

 

pLd7

repB

Replication initiator protein

 

orf2

Replication-associated protein

 

hsdR

Type I restriction-modification system specificity subunit R

 

hsdM

Type I restriction-modification system specificity subunit M

 

hsdS

Type I restriction-modification system specificity subunit S

 

corA

Magnesium transporter

 

tra

Transposase IS982 family

 

orf8

Hypothetical protein

 

orf9

Hypothetical protein

 

orf10

Hypothetical protein

 

tnpR

Resolvase

 

tra

Transposase IS6 family

 

orf13

MFS transporter

 

orf14

Acetyltransferase

 

orf15

Polysaccharide biosynthesis protein

 

orf16

Hypothetical protein

 

tra

Transposase IS6 family

 

dld

D-Lactate dehydrogenase

 

orf19

Hypothetical protein

 

orf20

Hypothetical protein

 

orf21

Hypothetical protein

 

tra*

Transposase IS6 family

 

uspA

Universal stress protein

 

mntH

Manganese transporter

 

tra*

Transposase IS3 family

 

orf26*

integrase/recombinase

 

orf27

Hypothetical protein

 

mobC

Mobilisation protein

 

mobA

Mobilisation protein

 

mobB

Mobilisation protein

 

mobD

Mobilisation protein

 

pLd8

repB1

Replication initiator protein

 

parA

Chromosome partitioning protein

 

orf3

Hypothetical protein

 

orf4

Serine protease

 

repB2

Replication initiator protein

 

orf6

Hypothetical protein

 

orf7

Hypothetical protein

 

orf8

Hypothetical protein

 

orf9

Hypothetical protein

 

orf10

Nickase

 

tra

Transposase IS6 family

 

mntH

Manganese transporter

 

uspA

Universal stress protein

 

tra

Transposase IS6 family

 

tra*

Transposase IS6 family

 

orf16

AAC(3) family N-acetyltransferase

 

tra

Transposase IS982 family

 

orf18

C4-dicarboxylate ABC transporter

 

tra

Transposase IS982 family

 

orf20

Hypothetical protein

 

orf21

Hypothetical protein

 

orf22

Large-conductance mechanosensitive channel

 

lacX

Hypothetical protein

 

lacG

6-phospho-beta-galactosidase

 

lacE

PTS lactose transporter subunit IIBC

 

lacF

PTS lactose transporter subunit IIA

 

lacD

Tagatose 1,6-diphosphate aldolase

 

lacC

Tagatose-6-phosphate kinase

 

lacB

Galactose-6-phosphate isomerase subunit LacB

 

lacA

Galactose-6-phosphate isomerase subunit LacA

 

lacR*

Lactose repressor

 

tra*

Transposase IS3 family

 

orf33

Type II restriction modification system

Leuconostoc mesenteroides

orf34

Type II restriction modification system

Leuconostoc mesenteroides

orf35

Hypothetical protein

 

tnpR

Resolvase

 

orf37

Hypothetical protein

 

orf38

Hypothetical protein

 

pepO

Neutral endopeptidase

 

oppA

Peptide-binding protein

 

oppC

Peptide ABC transporter permease

 

oppB

Peptide ABC transporter permease

 

oppF

Oligopeptide transport ATP-binding protein

 

oppD

Oligopeptide transport ATP-binding protein

 

tra

Transposase IS982 family

 

pLd9

repB

Replication initiator protein

 

orf2

Hypothetical protein

 

hsdS

Type I restriction-modification system specificity subunit S

 

abi*

Abortive phage resistance protein

 

tra

Transposase IS6 family

 

dld

D-lactate dehydrogenase

 

tra

Transposase IS6 family

 

orf8

Hypothetical protein

 

orf9

MFS transporter

 

tra

Transposase IS6 family

 

tra*

Transposase IS982 family

 

orf12

Amidohydrolase of peptidase M20 family

 

orf13

Hypothetical protein

 

orf14

Site-specific integrase

 

orf15

Integrase-associated protein

 

pLd10

repB1

Replication initiator protein

 

orf2

Replication-associated protein

 

tra

Transposase IS982 family

 

epsR

XRE family transcriptional regulator

 

epsX

Polysaccharide biosynthesis protein

 

epsA

Tyrosine protein kinase transmembrane modulator

 

epsB

Tyrosine protein kinase

 

epsC

Tyrosine protein phosphatase

 

epsD

Undecaprenyl-phosphate galactosephosphotransferase

 

epsE

Group 1 glycosyltransferase

 

epsL

Exopolysaccharide biosynthesis protein

 

orfY

LytR family transcriptional regulator

 

tra*

Transposase IS6 family

 

tra

Transposase IS3 family

 

tra

Transposase IS3 family

 

tra

Transposase IS256 family

 

orf17

XRE family transcriptional regulator

 

orf18

Replication-associated protein

 

repB2

Replication initiator protein

 

mobD

Mobilisation protein

 

mobB

Mobilisation protein

 

mobA

Mobilisation protein

 

mobC

Mobilisation protein

 

orf24

Hypothetical protein

 

orf25

Integrase

 

orf26

Hypothetical protein

 

tra*

Transposase IS6 family

 

orf28

Hypothetical protein

 

epsG

Glycosyltransferase family 2 protein

 

epsH

Glycosyltransferase family 2 protein

 

tra

Transposase IS6 family

 

epsI*

Glycosyltransferase

 

wzxA

Flippase

 

tra

Transposase IS6 family

 

tra*

Transposase

Lactobacillus/Oenococcus/Enterococcus

tra*

Transposase

Lactobacillus

tra

Transposase IS30 family

Lactobacillus

wzxB

Flippase

Lactobacillus

wzy

Polymerase

Lactobacillus

orf40

Hypothetical protein

 

epsM

Glycosyltransferase family 2 protein

Lactobacillus

epsN

Glycosyltransferase

Lactobacillus

epsO

Glycosyltransferase family 1 protein

Lactobacillus

orf44

DUF4411 domain-containing protein

Lactobacillus plantarum

tra

Transposase ISL3 family

Lactobacillus

orf46

Hypothetical protein

Lactobacillus

orf47

Hypothetical protein

 

orf48

Hypothetical protein

 

orf49

Resolvase

 

pLd11

repB

Replication initiator protein

 

orf2

Replication-associated protein

 

orf3

Relaxase/mobilisation nuclease domain protein

 

pepE

Peptidase E

 

tra

Transposase IS6 family

 

tra*

Transposase IS6 family

 

orf7

DUF1919 domain-containing protein

 

orf8

DUF3883 domain-containing protein

 

orf9

Relaxase

 

orf10

Hypothetical protein

 

orf11

Hypothetical protein

 

mobC

Mobilisation relaxosome protein

 

pLd12

repB

Replication initiator protein

 

hsdS

Type I restriction-modification system specificity subunit S

 
  1. Putative functions that could be beneficial in the dairy environment are shown in bold. Pseudogenes are indicated with an asterisk. The species that contains the best homolog is given of genes that are rarely found in L. lactis