Skip to main content

Table 3 Variants of Lactococcus lactis FM03P with different plasmids contents that were obtained in this study

From: Large plasmidome of dairy Lactococcus lactis subsp. lactis biovar diacetylactis FM03P encodes technological functions and appears highly unstable

L. lactis variant Cured plasmids Plasmid content Isolated from
FM03P pLd1,2,3,4,5,6,7,8,9,10,11,12 Samsø cheese
3 pLd4,5,10 pLd1,2,3,6,7,8,9,11,12 Protoplast-induced curing
5 pLd4,10,11 pLd1,2,3,5,6,7,8,9,12 Protoplast-induced curing
47 pLd8,11 pLd1,2,3,4,5,6,7,9,10,12 Overnight culture
48 pLd2,4,5,6,7 pLd1,3,8,9,10,11,12 Chemostat culture
49 pLd2,3,4,5,6,7 pLd1,8,9,10,11,12 Protoplast-induced curing
50 pLd4,5,6,7 pLd1,2,3,8,9,10,11,12 Protoplast-induced curing
51 pLd4,5,6,7,8,9,11,12 pLd1,2,3,10 Protoplast-induced curing
63 pLd3,4,8,11,12 pLd1,2,5,6,7,9,10 Protoplast-induced curing
FM03-V1 pLd7 pLd1,2,3,4,5,6,8,9,10,11,12 Chemostat culture
  1. Variants 47, 48 and FM03-V1 were isolated by plating on LM17 agar plates