Table 3 Variants of Lactococcus lactis FM03P with different plasmids contents that were obtained in this study
L. lactis variant | Cured plasmids | Plasmid content | Isolated from |
|---|---|---|---|
FM03P | – | pLd1,2,3,4,5,6,7,8,9,10,11,12 | Samsø cheese |
3 | pLd4,5,10 | pLd1,2,3,6,7,8,9,11,12 | Protoplast-induced curing |
5 | pLd4,10,11 | pLd1,2,3,5,6,7,8,9,12 | Protoplast-induced curing |
47 | pLd8,11 | pLd1,2,3,4,5,6,7,9,10,12 | Overnight culture |
48 | pLd2,4,5,6,7 | pLd1,3,8,9,10,11,12 | Chemostat culture |
49 | pLd2,3,4,5,6,7 | pLd1,8,9,10,11,12 | Protoplast-induced curing |
50 | pLd4,5,6,7 | pLd1,2,3,8,9,10,11,12 | Protoplast-induced curing |
51 | pLd4,5,6,7,8,9,11,12 | pLd1,2,3,10 | Protoplast-induced curing |
63 | pLd3,4,8,11,12 | pLd1,2,5,6,7,9,10 | Protoplast-induced curing |
FM03-V1 | pLd7 | pLd1,2,3,4,5,6,8,9,10,11,12 | Chemostat culture |