Large plasmidome of dairy Lactococcus lactis subsp. lactis biovar diacetylactis FM03P encodes technological functions and appears highly unstable

Table 4 Primer sequences used to detect plasmids in L. lactis FM03P

Target	Sequence (5′-- > 3′)		Amplicon	Tm (°C)
Chromosome	Fw	TTAATTCAACCTGGAGACACAGTCTTAG	254	65.2
Chromosome	Rv	CTATCAGCGATTTCACGGAACTTAG	254	65.6
pLd1	Fw	GCATTGACGGCTGTTGTAAT	209	62.5
pLd1	Rv	AGCAGATTCCCGAGGATAAC	209	62.0
pLd2	Fw	AATGGGCCGAAGGTTCTATT	285	63.4
pLd2	Rv	CAGGAACCGATTCTCCTGTTA	285	62.9
pLd3	Fw	CCTCTCGCGTTCCTTGATA	417	62.9
pLd3	Rv	CCACGTAAGGGCGATTTAGT	417	62.7
pLd4	Fw	GCGGTAACAACATCCGTATC	508	61.8
pLd4	Rv	AGTCAGCCCAAGCGACTAAT	508	62.7
pLd5	Fw	AAATACAAGTGTTGAAGGCGTTG	589	63.8
pLd5	Rv	ACCTTTGTTCTCCAATTTCAGC	589	62.2
pLd6	Fw	TAAGTGCAACTAAAAGAAATAATAAAGTGCAA	200	65.1
pLd6	Rv	TTGCTGATGATTGTACCAGCTAAAAC	200	65.8
pLd7	Fw	TGGGCATCTAGATAATCTGACGACATCTGT	596	71.3
pLd7	Rv	CGACATTGACTCCCCAAAAACCAAAAATGA	596	75.3
pLd8	Fw	CCCAGTTGATTTAGAATTAGCTGAAGAATA	588	65.8
pLd8	Rv	AGATAGGTTGCATCCAAGATAAATTTGTTA	588	65.9
pLd9	Fw	TAGTCGCTGGCAAATTTTACAATCA	323	67.0
pLd9	Rv	CTTTGGGGGTTGCTTTAGAATCAAT	323	67.5
pLd10	Fw	ACGCTTGAACCCCATCTTGG	255	68.3
	Rv	TCGTCCCAAACGGTTTACCC	255	67.8
pLd11	Fw	TTCAATGAATGGCTCGGAAGAA	388	67.6
	Rv	TTTCGGCACAGGAGCAACAT	388	67.9
pLd12	Fw	GAAAACTAATCTAGTACAATCATCAGCAAACTT	277	65.1
	Rv	TTTTTACGAGTTATATTGTTTCTAGTCAGATTCTT	277	64.7

Primers were used to determine the plasmid content of different variants and to discriminate variants in competition experiments

ISSN: 1471-2164