Table 4 Primer sequences used to detect plasmids in L. lactis FM03P
Target | Sequence (5′-- > 3′) | Amplicon | Tm (°C) | |
|---|---|---|---|---|
Chromosome | Fw | TTAATTCAACCTGGAGACACAGTCTTAG | 254 | 65.2 |
Rv | CTATCAGCGATTTCACGGAACTTAG | 65.6 | ||
pLd1 | Fw | GCATTGACGGCTGTTGTAAT | 209 | 62.5 |
Rv | AGCAGATTCCCGAGGATAAC | 62.0 | ||
pLd2 | Fw | AATGGGCCGAAGGTTCTATT | 285 | 63.4 |
Rv | CAGGAACCGATTCTCCTGTTA | 62.9 | ||
pLd3 | Fw | CCTCTCGCGTTCCTTGATA | 417 | 62.9 |
Rv | CCACGTAAGGGCGATTTAGT | 62.7 | ||
pLd4 | Fw | GCGGTAACAACATCCGTATC | 508 | 61.8 |
Rv | AGTCAGCCCAAGCGACTAAT | 62.7 | ||
pLd5 | Fw | AAATACAAGTGTTGAAGGCGTTG | 589 | 63.8 |
Rv | ACCTTTGTTCTCCAATTTCAGC | 62.2 | ||
pLd6 | Fw | TAAGTGCAACTAAAAGAAATAATAAAGTGCAA | 200 | 65.1 |
Rv | TTGCTGATGATTGTACCAGCTAAAAC | 65.8 | ||
pLd7 | Fw | TGGGCATCTAGATAATCTGACGACATCTGT | 596 | 71.3 |
Rv | CGACATTGACTCCCCAAAAACCAAAAATGA | 75.3 | ||
pLd8 | Fw | CCCAGTTGATTTAGAATTAGCTGAAGAATA | 588 | 65.8 |
Rv | AGATAGGTTGCATCCAAGATAAATTTGTTA | 65.9 | ||
pLd9 | Fw | TAGTCGCTGGCAAATTTTACAATCA | 323 | 67.0 |
Rv | CTTTGGGGGTTGCTTTAGAATCAAT | 67.5 | ||
pLd10 | Fw | ACGCTTGAACCCCATCTTGG | 255 | 68.3 |
| Â | Rv | TCGTCCCAAACGGTTTACCC | 67.8 | |
pLd11 | Fw | TTCAATGAATGGCTCGGAAGAA | 388 | 67.6 |
| Â | Rv | TTTCGGCACAGGAGCAACAT | 67.9 | |
pLd12 | Fw | GAAAACTAATCTAGTACAATCATCAGCAAACTT | 277 | 65.1 |
| Â | Rv | TTTTTACGAGTTATATTGTTTCTAGTCAGATTCTT | 64.7 | |