Fig. 2

GSIS and intracellular insulin content in isolated human islets exposed to palmitate for 0, 4 and 12 h and 1, 2 and 7 days. After culture, islets were perifused with 2 mM glucose followed by 20 min perifusion with 20 mM glucose. Panel a. Representative graphs of dynamic insulin secretion from one donor. Panel b. Insulin secretory rate at stimulatory (20 mM) glucose was normalized to total protein. Insulin secretion was expressed as a ratio between insulin secretory rate at stimulatory glucose from treated islets and insulin secretory rate at stimulatory glucose from untreated islets for each donor. Panel c. Islets were subsequently lysed and insulin content measured. Insulin content after treatments was normalized to total protein content and expressed as fold insulin content in untreated islets. Results are means of 5 donors ± SEM. *p < 0.05 vs control