Fig. 4From: The inconsistent regulation of HOXC13 on different keratins and the regulation mechanism on HOXC13 in cashmere goat (Capra hircus)HOXC13 had an inconsistent regulation on the promoters of KRT1 and KRT2 compared with KRT84 and KRT38. a-d The regulation of HOXC13 on keratin promoters including KRT1, KRT2, KRT38 and KRT84 were detected. The corresponding KRT promoter-pGL3 basic vector, pRL-TK vector together with HOXC13 overexpression vector (wtHOXC13/ΔHOXC13) were co-transfected into 12-well dermal papilla cells, while the vector of ΔHOXC13 was used as a negative control. * P < 0.05, **P < 0.01 (e-f) The regulation function of HOXC13 on keratin promoters including KRT2 and KRT84 were detected using KRT-promoter-GFP system. In this system, the CMV promoter before GFP was taken place by the promoter of KRT. The corresponding KRT promoter-GFP vector together with HOXC13 overexpression vector (wtHOXC13/ΔHOXC13) were co-transfected into 12-well HEK 293 T cells while the vector of ΔHOXC13 was used as a negative control. All the groups were performed in triplicate. The data was expressed as the mean ± 1 SE (n = 3). * P < 0.05, ** P < 0.01Back to article page