Fig. 5

The negative-feedback regulation of HOXC13 at transcriptional level. a Optimal promoter of HOXC13 was detected using PGL3-basic system in dermal papilla cells. b–c The negative-feedback regulation of HOXC13 at transcriptional level. HOXC13 overexpression vector together with HOXC13 p365-PGL3 basic vector were transfected into dermal papilla cells, while ΔHOXC13 / HOXC13 mut was used as a negative control. HOXC13P365 mut was a negative control to verify the binding site of HOXC13 in the promoter of HOXC13 itself. The data was expressed as the mean ± 1 SE (n = 3). * P < 0.05, ** P < 0.01