Fig. 9

SNPs in the homeodomain of HOXC13 affect its function on keratins. a Representation of HOXC13 and the position of the mutation (c.812A > G and c.929A > C) within the highly conserved DNA-binding domain. b-c SNPs in the homeodomain of HOXC13 affect its regulation on the promoter of KRT2 and KRT38. The specific KRT promoter-PGL3 basic vector together with wild type HOXC13 overexpression vector or mutational type overexpression vector were transfected into HEK 293 T cells, then the dual luciferase activity were detected. d SNPs in the homeodomain of HOXC13 affect its regulation on the promoter of KRT84 using KRT-promoter-GFP system in dermal papilla cells. e Western blot showed the relative protein level of HOXC13. The total protein was extracted from transfected HEK 293 T cells, blank HEK 293 T cells and skin sample from mouse, while blank cells and the mouse skin sample were used as a positive control