|
Assembly Statistics
|
|
Assembly
|
PnteEviGene
|
Pnte_v1.0
|
PiptEviGene
|
Pipt_v2.0
|
|
Total Sequences
|
91,552
|
28,621
|
325,974
|
52,735
|
|
Total Transcriptome Length (Mb)
|
100.95
|
50.01
|
259.02
|
72.15
|
|
Average Sequence Length (nt)
|
1103
|
1747
|
794
|
1368
|
|
N50 (nt)
|
1551
|
2296
|
870
|
1897
|
|
Longest Sequence (nt)
|
16,886
|
16,886
|
16,570
|
16,570
|
|
Shortest Sequence (nt)
|
351
|
351
|
351
|
351
|
|
%GC
|
48%
|
44%
|
50%
|
46%
|
|
Sequence filteringa
|
|
Sequences with FPKM > 1
|
77,563
| |
203,996
| |
|
(84.72%)
|
(62.58%)
|
|
Sequences with GeneMarkS-T predicted CDS
|
74,556
|
194,568
|
|
(81.44%)
|
(59.69%)
|
|
Total proteins after clustering to 90% identity
|
70,748
|
167,961
|
|
(77.28%)
|
(51.53%)
|
|
Annotationb
|
| |
Predicted protein frame
|
|
Complete
|
32,193
|
18,971
|
61,134
|
26,835
|
|
(45.50%)
|
(66.27%)
|
(36.40%)
|
(50.89%)
|
|
Internal
|
18,279
|
3347
|
57,027
|
9602
|
|
(25.84%)
|
(11.70%)
|
(33.95%)
|
(18.21%)
|
|
3′-partial
|
6686
|
1895
|
21,848
|
4354
|
|
(9.45%)
|
(6.63%)
|
(13.01%)
|
(8.26%)
|
|
5′-partial
|
17,398
|
4408
|
54,559
|
11,944
|
|
(24.59%)
|
(15.40%)
|
(32.48%)
|
(22.65%)
|
| |
Similarity Search Annotation
|
|
Sequences with informative BLASTp alignments
|
19,296
|
15,192
|
38,714
|
27,328
|
|
(27.27%)
|
(53.09%)
|
(23.05%)
|
(51.82%)
|
|
Sequences with uninformative BLASTp alignments
|
15,437
|
6131
|
34,101
|
9261
|
|
(21.82%)
|
(21.41%)
|
(20.30%)
|
(17.56%)
|
|
Sequences without BLASTp alignments
|
36,015
|
7300
|
95,146
|
16,147
|
|
(50.91%)
|
(25.50%)
|
(56.65%)
|
(30.62%)
|
| |
Functional Annotation
|
|
Sequences with family assignment
|
55,627
|
28,484
|
128,952
|
52,166
|
|
(78.63%)
|
(99.52%)
|
(76.77%)
|
(98.92%)
|
|
Sequences with at least one GO term
|
31,640
|
16,197
|
77,063
|
33,712
|
|
(44.72%)
|
(56.60%)
|
(45.88%)
|
(63.93%)
|
|
Sequences with at least one pathway (KEGG) assignment
|
17,303
|
8004
|
47,383
|
21,094
|
|
(24.46%)
|
(27.98%)
|
(28.21%)
|
(40.00%)
|
| |
Annotation Summary
|
|
Unannotated Sequences
|
14,568
|
0
|
36,698
|
0
|
|
(20.59%)
|
(0.00%)
|
(21.85%)
|
(0.00%)
|
|
Total sequences annotated
|
56,180
|
28,621
|
131,263
|
52,735
|
|
(79.41%)
|
(100.00%)
|
(78.15%)
|
(100.00%)
|
|
Non-pine origin sequences
|
27,550
|
0
|
78,527
|
0
|
|
(38.94%)
|
(0.00%)
|
(46.75%)
|
(0.00%)
|
