Whole genome sequencing reveals the emergence of a Pseudomonas aeruginosa shared strain sub-lineage among patients treated within a single cystic fibrosis centre

Table 1 Comparison of deletion events larger than 10 Kb in the AUST-02 genomes

Size (Kbp)	PAO1 locus tags	Features		Genome (strain/clade; patient ID; collection year)
Size (Kbp)	PAO1 locus tags	Gene	Function	Absent	Present
50–93	PA2165-PA2217	• exoY • hcnABC • cupA1-A5	T3SS secreted toxin Hydrogen cyanide biosynthesis operon Chaperone usher fimbrae operon	• AUS947 (M3L7; Patient 39; 2011) • AUS970 (M3L1; Patient 43; 2007) • AUS853 (M3 clade; Patient 45; 2007) • AUS854 (M3 clade; Patient 44; 2007)	• All other AUST-02 isolates
40	PA3619-PA3620	• Adjacent to mutS	Putative prophage	• AUS958 (M3L7; Patent 29; 2011) • AUS970 (M3L1; Patient 43; 2007) • AUS853 (M3 clade; Patient 45; 2007) • AUS854 (M3 clade; Patient 44; 2007)	• All other M3L7 isolates • Some Perth isolates^a • AUS24 (M2 clade; Patient 16; 2007)
14	PA1914- PA1923	• PA1914 • nrdD • PA1922	Encodes halovibrin Pathogenesis related factor TonB-dependent receptor	• All M3L7 isolates	• All other AUST-02 isolates
12	PA2229- PA2237	• pslABCDEFG	Biofilm formation	• All M3L7 isolates • AUS853 (M3 clade; Patient 45; 2007)	• All other AUST-02 isolates

^aAUS15 (M2 clade; Patient 18; 2008); AUS874 (M2 clade; Patient 22; 2008); AUS17 (M2 clade; Patient 17; 2008); AUS876 (M2 clade; Patient 21; 2008); AUS877 (M2 clade; Patient 20; 2008)

ISSN: 1471-2164