Fig. 2

Characterization of the mitochondrial sncRNA populations in male PGCs, spermatogonia and spermatozoa, and in oocytes and zygotes. a Read length distribution of mitochondrial derived sncRNAs from different cell types. The percentage of reads was calculated from the total reads in the small RNA-Seq library. b Classification of mitosRNAs in microRNAs (miRNA - mito-miRNAs), PIWI-interacting RNAs (piRNAs - mito-piRNAs) and sequences from non-coding RNAs present in the Ensembl database (ncRNAs). Reads that do not map to previous databases are considered as not annotated. c The percentage of mitochondrial encoded sncRNAs that map to the mouse genome (MM10 - including nuclear mitochondrial sequences) and those exclusive to mitochondrial DNA (MT). d Chromosome distribution of the mitosRNAs derived from the mouse genome. Normalization of the read count was carried out using DESeq. Male cells: primordial germ cells (PGCs), spermatogonia cells (SPG), and spermatozoa (SPZ). Female cells: oocytes (OCY) and zygotes (ZYGO)