Fig. 3

Analysis of the miRNAs derived from mtDNA in male PGCs, spermatogonia, spermatozoa, and in oocytes and zygotes. a Non-supervised hierarchical clustering of miRNA and miRNA isoform counts associated with the mitochondrial genome in the different samples. Read counts were normalized using DESeq and transformed to a base two logarithm. The annotation corresponds to the pre-microRNA and the miRNA isoforms. b Distribution of the different isoforms of miRNAs derived from mtDNA. MicroRNA isoforms: isomiR, miRNA isoforms with the same seed sequence as canonical and trimmed nucleotides in the 3′ region; paramiR, miRNA isoform where the 5′ region corresponds to the canonical miRNA sequences; and circumiR, the miRNA isoform where the 3′ region corresponds to the canonical miRNA sequences. c Different isomiRs and paramiRs found in miR-6390 in the different cell types: red bar corresponds to the canonical form of the miR-6390; the asterisks indicate the most expressed miRNA isoform in each sample. d Enrichment of KEEG pathways among the miR-6390 targets. Male cells: primordial germ cells (PGCs), spermatogonia cells (SPG), and spermatozoa (SPZ). Female cells: oocytes (OCY) and zygotes (ZYGO)