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Fig. 3 | BMC Genomics

Fig. 3

From: CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus

Fig. 3

Validation of homologous recombination at the Eef1a locus. a Schematic of the expected sequence for the edited region of Eef1aFLAG-P2A-eGFP. Exons are abbreviated E3 and E4. Sequence matching the repair template is shown in gray box. PCR primers are shown as small arrows. Coding sequence is colored as in Fig. 1. b Amplification of upstream and downstream regions of Eef1aFLAG-P2A-eGFP from eGFP+ and eGFP colonies

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