Fig. 8
From: CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus
Use of the StrepII-FLAG tag in immunoblots and pull-downs. a Western blot for FLAG on total protein lysates from Eef1aFLAG-P2A-tdTom transgenic and nontransgenic colonies. Sizes in italics are the top and bottom bands of the molecular size marker. b Western blot of StrepII tag on total protein lysates from the same colonies. The blot was treated to induce chemiluminescence and exposed to film for 5 min (left), followed by a second exposure for 60 min (right). The 15 kDa band suggests this antibody cross-reacts with some variants of an endogenous Hydractinia protein. Arrowhead indicates non-specific staining of protein ladder. c Coomassie stained SDS-PAGE gel of elutions from Strep-Tactin coated beads following incubation in total protein lysates from the indicated colonies. d Western blot against the FLAG peptide on the indicated amount of total protein lysate from colony 355–8, before and after incubation with the anti-streptavidin beads. The elution from the beads was run alongside for comparison