Immunomodulatory effects of heat stress and lipopolysaccharide on the bursal transcriptome in two distinct chicken lines

Table 3 Interactions between heat stress and LPS differed between lines

Type of DE Interaction^a	Significant			Number of Genes
	Inter-treatment^b	Single treatment^c	Heat + LPS^d	Heat + LPS to Heat + Saline		Heat + LPS to Thermoneutral + LPS
	Inter-treatment^b	Single treatment^c	Heat + LPS^d	Broiler	Fayoumi	Broiler	Fayoumi
Suppressed	Y	Y	N/Y	1	0	0	199
Unaffected	N	Y	Y	0	40	40	27
Induced	Y	N	Y	95	22	1	529

^aInter-treatment comparisons (Heat + LPS to Heat + Saline or to Thermoneutral + LPS) identified three effects of combined treatment on DE (suppressed, unaffected, or induced). See Additional file 6 for examples of each interaction type
^bHeat + LPS to Heat + Saline or Heat + LPS to Thermoneutral + LPS
^cHeat + Saline to Thermoneutral + Saline or Thermoneutral + LPS to Thermoneutral + Saline
^dHeat + LPS to Thermoneutral + Saline
Differential expression (DE), lipopolysaccharide (LPS), yes (Y), no (N)

ISSN: 1471-2164