Fig. 5

Real-time reverse transcription polymerase chain reaction (RT-PCR) verification of the RNA-Seq results. Data for comparison between the “ischemia–reperfusion” and “sham–operation” groups at 24 h after operation (IR24 vs. SH24) are shown. Two reference genes Gapdh and Rpl3 were used to normalize PCR-results. In each group, there were at least 9 rats. Eighteen genes that change the expression of more than 1.5-fold from the baseline value and whose P-value lower 0.05, as well as two other genes were selected for analysis