Skip to main content

Table 1 Alignment of contigs from different data sets to the AGPv2 reference genome using BLAST. To be classified as aligned, contigs must match the reference with ≥95% identity

From: Linked read technology for assembling large complex and polyploid genomes

No. Alignments

No. Contigs (%) from

LR Contigs

Trimmed LR Contigs

MAGIs

Sim 1a

Sim 2b

Sim 3c

ABySS

1 (Unique)

222,531 (95.0)

222,245 (95.1)

109,665 (96.1)

256,255 (98.7)

262,727 (98.8)

257,049 (98.7)

4,614,334 (42.9)

2

2145 (0.9)

2194 (0.9)

1347 (1.2)

1985 (0.8)

2014 (0.8)

1971 (0.8)

1,520,610 (14.1)

>  2

629 (0.3)

535 (0.2)

138 (0.1)

568 (0.2)

488 (0.2)

716 (0.3)

4,268,325 (39.7)

0

8848 (3.8)

8931 (3.8)

3024 (2.6)

762 (0.3)

719 (0.3)

790 (0.3)

350,313 (3.26)

Total

234,153

233,905

114,173

259,570

265,968

260,526

10,753,582

  1. aSimulation 1: 50 kb molecule length and 400 M reads
  2. bSimulation 2: 80 kb molecule length and 400 M reads
  3. cSimulation 3: 50 kb molecule length and 800 M reads