Genomic insights into host adaptation between the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei)

Table 1 Genome statistics of Puccinia striiformis isolates Pst (93–210) and Psh (93TX-2) in comparison with previously sequenced isolates^a

	Pst (93–210)	Psh (93TX-2)	Pst 104E 137A-	Pst 2 K-41-Yr9	Pst-CY32
Assembly statistics
Genome size in contigs (bp)	84,627,802	77,368,122	83,355,616	79,310,008	115,495,157
GC-content (%)	44.39	44.40	44.40	44.43	44.76
Number of contigs	493	562	156	17,295	10,913
Number of contigs > 100 kb	243	263	93	3	25
Contig N50	83	110	23	1320	1434
Contig N50 length (bp)	295,440	218,468	1,304,018	17,361	21,648
Mean contig size (bp)	171,659	137,666	534,331	4586	10,583
Annotation statistics
Protein-coding genes	16,513	15,976	15,928	19,912	25288^b
Annotation completeness^c (%)	95.10	93.00	97.60	98.60	94^b
Secreted proteins	1517	1624	2059	2056	2092^b
tRNA genes	648	626	636	668	862
Transposable elements (Mb)	30.46	26.41	31.08	24.87	37.68
Transposable elements (%)	36.03	34.2	37.28	31.35	32.62

^aStatistics were calculated using the latest references and annotations except noted elsewhere
^bData was directly retrieved from Zheng et al. (2013) as the annotation of Pst-CY32 was not publicly available at the time of writing this paper
^cProgram BUSCO [95] was used to evaluate the annotation completeness of assembled genomes

ISSN: 1471-2164