Fig. 5

Relative expression level of TRP channels in different developmental stages of B. dorsalis, including eggs (one and two hours old), larvae (one day, four days, and seven days old), pupae (one day, four days, and seven days old), one day old immature male and female adults, and 13 day old mature male and female adults. The data presented here are from two independent experiments performed in triplicate. The standard error is represented by the error bar and different letters above each bar denote significant differences (p < 0.05) according to ANOVA followed by Tukey’s multiple comparison test. Asterisks indicate values significantly different between the two splice forms of Pyx using unpaired t test (p < 0.05)