Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates

Table 4 Discrimination and information parameters for each microsatellite markers. The Simpson index and the observed heterozygosis obtained in the original procedure by Sabino et al. [5] are also included

Parameters	CP1	CP4	CP6	B5	Multiplex	Study
Polymorphic information content (PIC)	0.692	0.870	0.942	0.802	0.957	This study (2016)
Simpson Index	0.724	0.879	0.944	0.816	0.959
Observed heterozygosis	0.723	0.273	0.606	0.333	0.958
Unbiased estimation of heterozygosis	0.724	0.880	0.945	0.816	0.960
Entropy (in bits)	2.450	3.647	4.430	3.179	4.688
Simpson Index	0.850	0.890	0.960	0.860	0.990	Sabino et al. (2010) [5]
Observed heterozygosis	0.733	0.214	0.523	0.300	–	Sabino et al. (2010) [5]

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