A comparative analysis of library prep approaches for sequencing low input translatome samples

BMC Genomics

Table 3 Descriptive statistic of non-coding detected features (CPM > =1)

Kit	Sample type	Total features of ribosomal RNA			Average CPM of ribosomal RNA			Total features of ribosomal RNA, lincRNA, microRNA			Average CPM of ribosomal RNA, lincRNA, microRNA
		Replicate			Replicate			Replicate			Replicate
		1	2	3	1	2	3	1	2	3	1	2	3
NEB	Input	3	3	3	73.87	67.33	72.15	233	186	140	46.01	29.22	28.34
NEB	IP	3	3	4	14.24	6.15	7.80	128	157	268	78.05	28.33	34.25
NuG	Input	8	11	10	5533.70	5571.81	5603.65	552	488	415	272.73	274.17	276.54
NuG	IP	13	11	17	1803.34	820.73	863.91	583	593	637	97.91	48.26	51.37
SMTer	Input	8	5	6	4282.62	7858.83	4641.15	440	359	377	247.75	429.65	261.04
SMTer	IP	12	6	17	4880.27	7906.14	3936.78	467	411	599	282.51	429.64	240.08
Tru4	Input	17	17	13	232.50	120.94	169.47	804	786	767	13.96	7.59	10.29
Tru4	IP	8	7	19	415.93	129.19	168.59	546	650	927	26.49	8.94	11.45
Tru70	Input	6	4	4	102.39	28.55	58.50	410	363	406	6.34	2.08	3.81
Tru70	IP	3	3	4	141.53	54.91	65.21	265	295	341	9.24	3.97	5.10
SMTseq4	Input	13	13	21	214.51	159.76	185.17	750	764	803	11.78	8.93	10.54
SMTseq4	IP	7	11	10	851.62	63.75	92.92	844	881	889	46.39	5.91	8.73
SMTseq0.25	Input	6	7	3	224.50	191.46	235.20	531	515	461	12.32	10.52	13.04
SMTseq0.25	IP	6	6	7	886.27	73.72	99.28	476	502	457	48.03	6.43	8.88

ISSN: 1471-2164