A comparative analysis of library prep approaches for sequencing low input translatome samples

BMC Genomics

Table 4 Descriptive statistic of protein-coding detected features (CPM ≥ 1)

Kit	Sample type	Total features of protein-coding RNA			Average CPM of protein-coding RNA
		Replicate			Replicate
		1	2	3	1	2	3
NEB	Input	11,316	10,551	9789	36.51	37.77	37.83
NEB	IP	8740	10,252	12,435	34.13	37.84	37.39
NuG	Input	10,921	10,828	10,656	19.61	19.50	19.32
NuG	IP	12,970	13,142	13,318	32.65	36.35	36.12
SMTer	Input	11,136	9368	10,019	21.47	7.90	20.48
SMTer	IP	11,826	11,860	11,824	18.88	7.90	22.02
Tru4	Input	11,826	11,860	11,824	38.90	39.38	39.18
Tru4	IP	12,360	12,842	12,941	37.97	39.28	39.09
Tru70	Input	11,690	11,691	11,611	39.47	39.79	39.66
Tru70	IP	11,996	12,493	12,660	39.26	39.65	39.56
SMTseq4	Input	11,659	11,499	11,578	39.07	39.28	39.16
SMTseq4	IP	12,443	12,579	12,587	36.50	39.51	39.01
SMTseq0.25	Input	11,500	11,475	11,384	39.03	39.16	38.97
SMTseq0.25	IP	12,648	12,846	12,940	36.38	39.47	39.29

ISSN: 1471-2164