Fig. 3

Heatmap of differentially expressed genes (DEGs) during limb regeneration in Bolitoglossa ramosi (Caudata: Plethodontidae). a DEGs with a ≥ 2-fold expression change and good transcriptional support (TPM ≥ 0.95) were considered (n = 602). Each column of the heatmap indicated the 2-fold changes of each sample respect the control limb, dpa: days post-amputation. Two clusters were identified that largely consisted of up-regulated (red clusters, n = 310) and down-regulated genes (green cluster, n = 292). b A group of genes exhibiting down-regulation at 20 dpa but up-regulation from 40 to 70 dpa (orange square in a). c A group of genes markedly up-regulated at 40 dpa (yellow Square in a). d ncRNAs identified as DEG, with the heatmap showing RFAM or miRBase ID