Fig. 1

Size estimation of the striped catfish genome and assessment of assembled genome. a Paired-end sequences in P. hypophthalmus were analyzed using GenomeScope software [78] . The estimated genome size was ~ 700 Mbp, based upon K-mer frequency (K = 41). b Assessment of the assembled genome was performed using BUSCO ver. 3. Comparisons with Benchmarking universal single-copy orthologs (BUSCO) sets representing 4584 genes for the Actinopterygii lineage indicated that 92.3% complete BUSCOs were detected in the draft genome, supporting the high quality of genome assembly. c Phylogenic position of the sequenced striped catfish was confirmed with an ML tree, which was constructed by comparing 10,665 bp encoding 12 mitochondrial protein genes of 112 species of the order Siluriformes deposited in the NCBI database. The mitochondrial genome sequence of our specimen was almost identical to that of P. hypophthalmus decoded in a previous study (NC_021752). The morphological identification of species is confirmed by COX1 gene sequences with voucher numbers (e.g., KU692728 and JF292409 in NCBI). The sister group relationship between two clades (Pangasiidae vs Cranoglanididae/Ictaluridae) was also supported by the previous study [102]. Nodes with white circles were supported by a partition analysis excluding 3rd codon positions (7110 bp). Divergence times between species with decoded genome (arrowheads) were obtained from TIMETREE (http://www.timetree.org): Danio vs Cyprinus (106 Mya), Danio vs Pangasianodon (144 Mya), and Ictalurus vs Pangasianodon (76 Mya)