Fig. 4

This figure provides the subcellular distribution of 149 hepatic AR-DEGs that regulate “Synthesis Lipid” from the contrast of all embryos vs. all hatchlings. Higher gene expression in embryos is indicated by red symbols and AR-DEGs with higher expression in liver of hatchlings have green symbols. However, the Ingenuity Upstream Regulator Analysis predicts that lipid synthesis would be inhibited in embryos as indicated by the blue label and blue dashed lines. This IPA prediction is based on actual E/H expression ratios and the observation that the number of AR-DEGs with higher expression in hatchlings (denominator) is 2-times greater than that of embryos (numerator). A small group of 14 transcription factors controls a much larger group of downstream metabolic genes. The largest number of AR-DEGs was found in “Cytoplasm”; these genes encode metabolic enzymes, kinases, phosphatases and transporters. The “Plasma Membrane” is mainly composed of genes encoding transmembrane and G-protein coupled receptors, transporters, enzymes, kinases and phosphatases. The extracellular AR-DEGs are a mixture of growth factors, adipokines, cytokines and transporters