Fig. 5

A gene network (Panel a) identified by IPA from the contrast of embryos (E) vs. hatchlings (H) that shows interaction of three lipogenic transcription factors (THRSPA, PPARG, SREBF2) with several direct target genes. This gene network was functionally annotated by IPA as involved in “Lipid Metabolism”. The legend provides a gene’s type while its relative expression (log2 ratio of E/H) indicates either higher expression in the embryo (red symbols) or a greater abundance in liver of hatchlings (green symbols). Panel b, Ingenuity® Upstream Regulator Analysis identified 28 direct targets of sterol response element binding factor 2 (SREBF2); most of which were expressed at higher levels in hatchlings. Ingenuity Upstream Regulator Analysis predicts inhibition of SREBF2, since 22 known direct targets of SREBF2 were downregulated (green symbols) in the E vs. H contrast. Actually, genes with green symbols are more abundant in liver of the hatchlings, since they have negative log2 E/H ratios