Table 5 Resources required for both technologies. Analysis was performed on 16 cores (Intel Xeon E5–2670 @ 2.60 GHz) with 64 GB of RAM Linux server running 64-bit CentOS distribution
Computing time | Memory requirements | Storage requirements | ||||
|---|---|---|---|---|---|---|
RNA-seq | HTA | RNA-seq | HTA | RNA-seq | HTA | |
Mapping to the transcriptome (STAR) | 11.5 h | – | 32Gb | – | 1023 GB | – |
Splicing graph generation (SGSeq) | 2 days (5 cores) | 14 h | 8 Gb per core | 5 Gb | 70.3 Mb | 2 Gb |
Event detection | 7 min 16 s (10 cores) | 1.2 Gb per core | 643.6 Mb | |||
Statistical analysis | 1 min 43 s | 3 min 06 s | 2 Gb | < 1Gb | 6.2 Mb | 11 Mb |