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Fig. 4 | BMC Genomics

Fig. 4

From: Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing

Fig. 4

Analyses of differentially expressed genes in 2–4 cell stage embryos. a PCA analysis of our data compared to GSE70608 in 4-cell NT embryos. Red crisscross (NT arrest): 4-cell embryos are developmentally arrested in the 4-cell stage (GSE70608). Green multiplication sign (NT to blast): 4-cell embryos develop to the blastula stage (GSE70608). Blue triangle: Our 4-cell NTC embryos. Purple circle: Our 4-cell NTM embryos. b Correlation analysis of our data compared to GSE70608 in 4-cell NT embryos. c Volcano plot of 2–4 cell stage embryos. Green dots indicate down-regulated genes in the 4-cell stage compared with the 2-cell stage, red dots indicate up-regulated genes in the 4-cell stage compared with the 2-cell stage, and gray dots indicate genes that did not display differences in transcription between the 4-cell and 2-cell stages. Every plots' Ensembl genes ID were listed in Additional file 7: Table S2. d Heatmap of all up-regulated genes (red dots) and down-regulated genes (green dots) shown in (a). Blue indicates lower expression, and red indicates higher expression. Three clustered genes were selected with a square. The meanings of the clusters are explained in Fig. 3. e GO analyses of clustered genes selected as described in (b)

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