Fig. 4
From: High-throughput proteomic profiling of the fish liver following bacterial infection
Response of the complement system in liver of AS-challenged rainbow trout. a Complement system pathway annotated with protein components quantified in our study. The numbers in parentheses highlight how many unique proteins were identified in our dataset for each complement component. The number of significantly upregulated complement proteins for each system component is shown by orange arrows. b Clustering of all identified complement system proteins; titles in bold show significantly differential abundance for AS (n = 6) vs. PBS (n = 5). c Maximum likelihood phylogenetic analysis of C3 proteins from: Human Homo sapiens (“Hs”), zebrafish Danio rerio (“Dr”), tilapia Oreochromis niloticus (“On”), northern pike Esox lucius (“El”, a sister lineage to salmonids that did not undergo the salmonid-specific whole genome duplication [53]), Atlantic salmon Salmo salar (“Ss”), and rainbow trout Oncorhynchus mykiss (“Om”). The tree is annotated to show potential teleost genome duplication events in the teleost (“3R”) and salmonid ancestor (“4R”). Branch support values are shown as circles on each node. Vertebrate C5 proteins provided a validated outgroup to vertebrate C3 proteins [55]. Abundance fold-change values for proteins identified in this study are displayed using bubble plots, with closed circles depicting significantly upregulated proteins