Fig. 3

Improved coverage of human and bacterial genomes with Nextera DNA Flex. a Coverage across important regions of the human genome by three library preparation kits: Nextera DNA Flex, TruSeq Nano, and TruSeq PCR-free. b Coverage across extreme regions of the human genome by three library preparation kits: Nextera DNA Flex, TruSeq Nano, and TruSeq PCR-free. c Libraries generated from the small genomes of bacteria with low (B. cereus), medium (E. coli), and high (R. sphaeroides) GC content at 1 ng inputs. Less coverage variation was observed with libraries prepared by Nextera DNA Flex compared with libraries prepared by other commercially available library preparation kits (NEB, NEBNext Ultra DNA Library Prep Kit; Kapa A, Kapa HyperPlus Kits; Kapa B, Kapa HyperPrep Kits), particularly for the low and high GC content genomes of B. cereus and R. sphaeroides. The method of DNA fragmentation and whether PCR amplification was used during library preparation is indicated