Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

BMC Genomics

Table 4 Libraries prepared from low DNA inputs of small genomes with low to high GC contents

Organism	Input DNA (ng)	PCR cycles (n)	Library yield (ng/μl)	Median fragment size (bp)	Median insert size (bp)	Mean coverage (X)	CV of coverage across the genome (%)
B. cereus (35% GC)	0.5	12	3.2	519	287	52.6	19.1
	1	10	1.9	518	289	53.1	19.2
	10	8	6.3	531	301	52.6	19.2
E. coli (51% GC)	0.5	12	5.0	577	304	61.9	16.7
	1	10	3.3	578	310	62.2	16.6
	10	8	8.6	581	308	62.2	16.9
R. sphaeroides (69% GC)	0.5	12	6.3	542	299	61.2	21.8
	1	10	3.6	533	301	61.6	21.8
	10	8	10.3	546	294	61.2	21.7

Libraries were prepared from 0.5, 1, or 10 ng bacterial DNA. Data was generated on a HiSeq 2500 Rapid Run (2 × 151 bp) and data analysis performed using BWA aligner application on BaseSpace and SAMtools. Fragment size was determined by Bioanalyzer. Insert size was determined during sequencing. CV Coefficient of variation. GC Guanine-cytosine

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