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Table 4 Libraries prepared from low DNA inputs of small genomes with low to high GC contents

From: Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

Organism Input DNA (ng) PCR cycles (n) Library yield (ng/μl) Median fragment size (bp) Median insert size (bp) Mean coverage (X) CV of coverage across the genome (%)
B. cereus (35% GC) 0.5 12 3.2 519 287 52.6 19.1
1 10 1.9 518 289 53.1 19.2
10 8 6.3 531 301 52.6 19.2
E. coli (51% GC) 0.5 12 5.0 577 304 61.9 16.7
1 10 3.3 578 310 62.2 16.6
10 8 8.6 581 308 62.2 16.9
R. sphaeroides (69% GC) 0.5 12 6.3 542 299 61.2 21.8
1 10 3.6 533 301 61.6 21.8
10 8 10.3 546 294 61.2 21.7
  1. Libraries were prepared from 0.5, 1, or 10 ng bacterial DNA. Data was generated on a HiSeq 2500 Rapid Run (2 × 151 bp) and data analysis performed using BWA aligner application on BaseSpace and SAMtools. Fragment size was determined by Bioanalyzer. Insert size was determined during sequencing. CV Coefficient of variation. GC Guanine-cytosine