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Table 5 Libraries prepared from human gDNA by manual preparation and two automated liquid handler platforms

From: Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

Metrics   Manual Preparation Hamilton NGS STAR™ Eppendorf epMotion® 5075 t
Yield (Qubit), ng/μl Run 1
Run 2
12.4–14.1
11.1–13.5
9.1–11.1
13.7–15.6
12.8–14.2
14.1–17.4
Yield CV, % Run 1
Run 2
5.1
6.2
8.3
4.8
3.5
7.3
Index CV, % Run 1
Run 2
10.8
12.1
13.7
11.7
11.0
12.6
Median insert size, bp
(optimal: 350 ± 50 bp)
Run 1
Run 2
348–357
350–363
375–391
377–385
335–344
351–368
Autosome mean coverage, X   30–32 30–32 30–32
Coverage across the human genome at 15X, %   97.6 97.7 97.4
Coverage across exonic regions at 10X, %   99.6 99.6 99.8
Mean diversity   > 2.0e9 > 2.0e9 > 2.0e9
Autosome Callability, %   95.0 94.5 94.5
  1. Libraries were prepared from 200 to 300 ng of human DNA (NA12878) using an Illumina Qualified method. Data is presented for two 8-plex runs on a HiSeqX system, with sequencing reads trimmed to a 30X depth (380 million reads, 2 × 151 bp). Data analysis performed using the BaseSpace Sequence Hub Whole Genome Sequencing v5.0 App. CV Coefficient of variation