Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

BMC Genomics

Table 5 Libraries prepared from human gDNA by manual preparation and two automated liquid handler platforms

Metrics		Manual Preparation	Hamilton NGS STAR™	Eppendorf epMotion® 5075 t
Yield (Qubit), ng/μl	Run 1 Run 2	12.4–14.1 11.1–13.5	9.1–11.1 13.7–15.6	12.8–14.2 14.1–17.4
Yield CV, %	Run 1 Run 2	5.1 6.2	8.3 4.8	3.5 7.3
Index CV, %	Run 1 Run 2	10.8 12.1	13.7 11.7	11.0 12.6
Median insert size, bp (optimal: 350 ± 50 bp)	Run 1 Run 2	348–357 350–363	375–391 377–385	335–344 351–368
Autosome mean coverage, X		30–32	30–32	30–32
Coverage across the human genome at 15X, %		97.6	97.7	97.4
Coverage across exonic regions at 10X, %		99.6	99.6	99.8
Mean diversity		> 2.0e⁹	> 2.0e⁹	> 2.0e⁹
Autosome Callability, %		95.0	94.5	94.5

Libraries were prepared from 200 to 300 ng of human DNA (NA12878) using an Illumina Qualified method. Data is presented for two 8-plex runs on a HiSeqX system, with sequencing reads trimmed to a 30X depth (380 million reads, 2 × 151 bp). Data analysis performed using the BaseSpace Sequence Hub Whole Genome Sequencing v5.0 App. CV Coefficient of variation

ISSN: 1471-2164