Fig. 3

Verification by qRT-PCR of DEGs and differential expressed miRNAs. GAPDH and U6 were used as reference genes. The expression levels of each mRNA and miRNA were normalized by comparison with their expression with BV stage. The abscissa represents the different stages, ordinate represents the relative expression. The bar with oblique stripes represents the relative expression base on qRT-PCR results and the bar with faillette base on sequencing results. Figure a-h indicated the expression of eight DEGs and fig. i-p indicated 8 differential expressed miRNAs, the bottom title represents the gene and miRNA name, respectively