Characterization and expression profiling of microRNAs in response to plant feeding in two host-plant strains of the lepidopteran pest Spodoptera frugiperda

Table 2 Experimental validation of variation in miRNA expression

Experimental condition	MicroRNA	Relative expression	Std Error	95% C.I.	P(H1)	Result
SfC (Rice/Corn)	miR-31 (Ref)	1
	miR-34	1.763	(1.080–3.062)	1.003–3.513	0.004	UP
	miR-190	1.549	(1.320–1825)	1.235–2.032	0.001	UP
SfR (Rice/Corn)	miR-31 (Ref)	1
	miR-190	1.218	0.671–1.520	0.900–1.669	0.05	UP
	tca-miR375*	5.802	(3.115–11,105)	1.511–14.148	0.002	UP
On Corn (SfR/SfC)	miR-31(Ref)	1
	miR-34	1.868	1.395–2.466	1.282–2.811	0.003	UP
	dme-miR-275*	0.965	0.821–1.121	0.726–1.251	0.587
On Rice (SfR/SfC)	miR-31(Ref)	1
On Rice (SfR/SfC)	dme-miR-275*	1.434	1.236–1.545	1.194–1.907	0.001	UP

The relative expression of miR genes depending on the host-plant (on rice compared to corn) in either the C or the R strain, or depending on the genetic background (SfR compared to SfC) was calculated according to [57]
P(H1) Probability of alternate hypothesis that difference between sample and control groups is due only to chance. The miRs with a star correspond to * Novel miRs having conserved seed region only

ISSN: 1471-2164