Fig. 1
From: Exposure to maternal obesity alters gene expression in the preimplantation ovine conceptus
The number of up- and down- regulated genes across experimental comparisons. Ovine conceptuses were collected from obese and lean ewes on day 14 of pregnancy via uterine flush. Conceptus sex was determined by PCR using X- and Y-specific primers, and 4 conceptuses/sex/treatment underwent RNA sequencing (N = 16 total conceptuses). Sequencing analysis was performed using Genomics Workbench 10.1.1 (CLC bio). Sequences were mapped to the Ovis aries genome (NCBI; Oar_4.0). Differentially expressed genes (DEGs) were identified as having a FDR ≤ 0.05, ≥ 2-fold change, and ≥ 0.2 RPKM. The number of DEGs that were up (gray) or down (black)-regulated is indicated based on obesity status, sex, and changes that were unique in individual comparisons between obese/lean versus male/female conceptuses