Fig. 2

Heat map showing clustering patterns for placenta-associated DEGs exhibiting sex and obesity-dependent effects. Ovine conceptuses were collected from obese and lean ewes on day 14 of pregnancy by uterine flush. Conceptus sex was determined by PCR using X- and Y-specific primers, and 4 conceptuses/sex/treatment underwent RNA sequencing (N = 16 total conceptuses). Sequencing analysis was performed using Genomics Workbench 10.1.1 (CLC bio). Sequences were mapped to the Ovis aries genome (NCBI; Oar_4.0), and differentially expressed genes (DEGs) were identified as having a FDR ≤ 0.05, ≥ 2-fold change, and ≥ 0.2 RPKM. Genes involved in placentation were identified from the list of DEGs via literature search. Individual gene IDs can be found in Additional file 6