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Fig. 2 | BMC Genomics

Fig. 2

From: Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors

Fig. 2

Molecular analysis of hop transgenic lines overexpressing HlWRKY1 and HlWRD1 transcription factors. (I) Schematic representation of the T-DNA region of the plant expression vector WWpPCV91, used for hop transformation.LB: left border, pmas: mannopine synthase bidirectional promoter, HPT: hygromycin phosphotransferase gene, e35S - enhancer of the 35S promoter from CaMV virus, pnos: nopaline synthase promoter, RB: right border. (II) Southern blot of hop genomic DNA isolated from WW-transgenic lines (B11, B23, B24) and control plants. The hybridization was performed with HPT (a) and WDR probe (b). The DNA markers (1 kb ladder, BRL) are positioned on the left sides

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