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Fig. 6 | BMC Genomics

Fig. 6

From: Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors

Fig. 6

Validation of RNA sequencing by RT-qPCR. Graph showing fold change of the structural and regulatory genes in a leaf of hop among transgenic lines (B11, B22, and B23) overexpressing HlWRKY1 and HlWDR1 transcription factors. PAL: phenylalanine ammonia lyase; C4H: cinnamate 4-hydroxylase, 4CL: coumarate coenzyme A ligase, CHS_H1: chalcone synthase isoform 1, PRT1: prenyltransferase 1; OMT1: O-methyltransferases isoform 1; VPS: valerophenone synthase, F3H: flavanone 3-hydroxylase. qRT-PCR analyses were normalized using GAPDH as an internal control gene. The fold change of each gene was calculated by the 2−ΔΔCT method. *Statistically significant differences (P < 0.05); **significant at p < 0.01

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