Fig. 7

Directly binding of miR-22 to ssc_circ_009380. The relative level of miR-22 in IPEC-J2 treated with (a) Schematic overview of dual-luciferase report plasmid. The locations of the putative binding sites or their mutations are presented by blank boxes. b Schematic overview of mutation of ssc_circRNA_009380 sequences. The upper sequences are the binding sites of miR-22. The middle sequences are the sequences of mature miR-22. The lower sequences are the mutated miR-22 binding sites. c miR-22 mimics and d miR-22inhibitors. e and f The relative luciferase activities of psi-ssc_circ_009380-WT and psi-ssc_circ_009380-Mut in response to miR-22 mimics and miR-22 inhibitors. ^*p < 0.05 in comparison with the control. ^**p < 0.01 in comparison with the control