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Table 2 Overview of transcriptome and small RNA sequencing in fresh and capacitated boar sperm

From: High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm

 

Data type

FS

CS

mRNA

Clean reads(pair-end)

26,843,452

29,925,873

Clean bases

6,642,110,360

7,481,508,592

% ≥ Q30

86.90

86.06

Total Reads

53,686,904

59,851,746

Mapped Reads(single-end)

30,016,749(55.91%)

33,084,783(55.28%)

Uniq Mapped Reads

28,565,403(53.21%)

30,691,568(51.28%)

miRNA

Raw reads

18,956,444

16,209,736

Clean reads

12,561,033

11,222,990

% ≥ Q30

86.43

87.25

Mapped Reads

3,027,230

2,944,033

Total-miRNAs

1028

1020

Known-miRNAs

259

238

novel-miRNAs

769

782

  1. Note: Clean reads (pair-end), the total number of pair-end reads in clean reads. Clean bases, the total number of bases in clean reads. ≥Q30, the percentage of the base that the quality value of clean data is greater than or equal to 30.Mapped reads (single-end), the number of bases for compared to the reference genome’ clean reads and the percentage in clean reads. Uniq Mapped Reads, the number of reads that are the only place in the reference genome and the percentage in clean reads. Known-miRNA, the number of miRNA is known. Novel-miRNAs, the number of new miRNA for predicted. Total-miRNAs, the number of total miRNA
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BMC Genomics

ISSN: 1471-2164

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