Table 4 Primers information of miRNAs and mRNAs for qRT-PCR validation
Gene ID | Primer sequences (5′-3′) | Amplicon (bp) | GenBank/miRBase accession |
|---|---|---|---|
PPIA | F:CACAAACGGTTCCCAGTTTT | 174 | NM_214353 |
R:TGTCCACAGTCAGCAATGGT | |||
PDHA1 | F:GATGATGCAGACTGTTCGCC | 138 | XM_003360244 |
R:TCCGTAGGGTTTATGCCAGC | |||
VDAC1 | F:TGATGGGACGGAGTTTGGTG | 115 | NM_213960 |
R:GGCTGCTATTCCAAAGCGTG | |||
CABYB | F:AAGTAGCTCACGGTCCTTCG | 202 | NM_001256771 |
R:GGCATACTTGTTGCCACATCC | |||
AKAP3 | F:GCACCCAACAAAAGCCTGAG | 96 | XM_021090980 |
R:GCCGGGAGTCTTATCCGAAG | |||
PGK1 | F:GCTGGACGTGAAGGGAAAGA | 104 | NM_001099932 |
R:CTGACTTGGCTCCGTTGTCT | |||
FSCB | F:GCTATTGATGAAGCAGCCCC | 74 | XM_001924913 |
R:AGTGAGTGTCTCCTTGGTGG | |||
PSPI | F:TGGGCCTTGCTGTTCAGT | 202 | NM_213837 |
R:TCCCACAGGTGAGGTTGAGA | |||
HSPA2 | F:TGAGCGGTACAAGTCGGAAG | 119 | XP_003356782 |
R:TCTTGCCCCTCAGTTTCTCG | |||
ssc-miR-127 | TCGGATCCGTCTGAGCTTGGCT | – | MI0013144 |
ssc-miR-1285 | CTGGGCAACATAGCGAGACCCCGT | – | MI0013164 |
ssc-miR-151-3p | CTAGACTGAAGCTCCTTGAGGA | – | MIMAT0013883 |
ssc-miR-152 | TCAGTGCATGACAGAACTTGG | – | MI0013104 |
conservative_1_2721 | ATTTGTGCTTGGCTCTGTCA | – | Novel |
conservative_7_221,178 | GGGGGGTGTAGCTCAGTGGTAGAGC | – | Novel |
conservative_7_234493 | GCTGGGTGCTGGCTGGGGC | – | Novel |
conservative_X_268567 | TGGCGGGCGGCGGGCGGCGGGC | – | Novel |
U6 | F:TTATGGGTCCTAGCCTGAC | – | EU520423 |
R:CACTATTGCGGGTCTGC |