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Fig. 6 | BMC Genomics

Fig. 6

From: Alterations in exosomal miRNA profile upon epithelial-mesenchymal transition in human lung cancer cell lines

Fig. 6

Effect of treatment with E/M-exosomes in 16HBE recipient cells. a Exosomes derived from 16HBE, A549 and H1299 were taken up by 16HBE cells. b Morphological cellular changes of epithelial 16HBE cells upon E/M-exosomes delivery. c The migrated cell numbers were significantly higher in the group with M1-exo treatment. d In matrigel invasion assays, more invaded cells were found in E1, M1 and M2-exo treatment groups. (Considering that 16HBE were non-tumor cells and were harder to pass through the chamber coated with matrigel, we extended the incubation time to 60 h). e E/M-exosomes induced an increase in N-cadherin in 16HBE cells. Protein levels of E-cadherin became more pronounced after co-culture with E2- and M2-exosomes than other groups. f E/M-exosomes from A549 cells affected the mRNA expression of EMT-related markers in 16HBE cells. The mRNA level of vimentin and snail were significantly upregulated after M-exosome stimulation, and M-exosomes showed stronger influence than E-exosomes. (*P < 0.05, **P < 0.01)

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