Fig. 1

Purification of terminally differentiated cell-types. a Schematic drawing of cell differentiation during Dictyostelium discoideum development. b Phase contrast image of spores isolated from dissociated fruiting bodies by sieving and detergent treatment to lyse amoebas. c Fruiting body stalks purified on a Percoll gradient. d Cells transformed with a gene fusion of the cup promoter DDB_G0278537 (beiB) and YFP were developed into fruiting bodies and imaged by fluorescence microscopy. Top: Phase contrast. Bottom: YFP fluorescence. Note the less opaque areas in the spore head, corresponding to upper and lower cup regions. e An example of FACS experiment with the cellular fraction of dissociated [beiB]:-YFP fruiting bodies. Scale bar = 100 μm