Genetic variants in the SIRT6 transcriptional regulatory region affect gene activity and carcass quality traits in indigenous Chinese beef cattle (Bos taurus)

Table 1 Primers used in this study

Name	Function	Primer Sequence (5′ to 3′)	Tm (°C)	Product Length	Amplified Region
SIRT6	qPCR	CAACCTGGAGAAATACCGTCTT	61.0	166 bp	400–565
SIRT6	qPCR	CAGTCCTTTTTCCTTCAGCAG	61.0	166 bp	400–565
β-actin	Reference	CACCAACTGGGACGACAT	61.0	202 bp	320–521
β-actin	Reference	ATACAGGGACAGCACAGC	61.0	202 bp	320–521
GAPDH	Reference	CCAACGTGTCTGTTGTGGAT	61.0	80 bp	778–857
GAPDH	Reference	CTGCTTCACCACCTTCTTGA	61.0	80 bp	778–857
Primer A	SNP detection	GAGACGGCCAGGAAGGAC	62.7	320 bp	71 – − 249
Primer A	SNP detection	CTGAACGAGGAAACAACG	62.7	320 bp	71 – − 249
Primer B	SNP detection	GACCCCTTCGTCCCCTCAAA	58.5	1367 bp	− 558 – − 1925
Primer B	SNP detection	GGGTGGGAAGAGCCAGTAGC	58.5	1367 bp	− 558 – − 1925
Primer- c.-84 C > T	SNP genotyping	GAGGTAAGTGGGCGTCAG	60.5	357 bp	− 312 – 45
Primer- c.-84 C > T	SNP genotyping	CACCAAAGGGAACAATAAAG	60.5	357 bp	− 312 – 45
Primer- c.-1100 A > G	SNP genotyping	CCTCAGCTCCCTCCCTCCTAC	62.7	148 bp	− 1218 – − 1070
Primer- c.-1100 A > G	SNP genotyping	CATGATCAGGTGTCAGGGTTGAAT	62.7	148 bp	− 1218 – − 1070

ISSN: 1471-2164