Metagenomic analysis of viral nucleic acid extraction methods in respiratory clinical samples

BMC Genomics

Table 5 Comparison of different extraction kits

Kit^a	Target	Specimen type	Requirement		Starting volume^c (μL)	Elution volume^c (μL)
Kit^a	Target	Specimen type	Reagents^b	special equipment	Starting volume^c (μL)	Elution volume^c (μL)
VRMK	viral RNA	Plasma, serum and cell-free body fluids	–	–	200	50
MVSK	viral RNA, viral DNA	Plasma, serum and cell-free body fluids	–	–	200	50
RPMK	RNA	cells, tissues	β-mercaptoethanol	gDNA Eliminator spin column	200^d	50^d
RMK	viral RNA	cells, tissues	β-mercaptoethanol	–	200	50

^aVRMK, QIAamp Viral RNA Mini Kit; MVSK, QIAamp MinElute Virus Spin Kit; RPMK, RNeasy Plus Micro Kit; RMK, RNeasy Mini Kit
^bNot included in the kit
^cIn order to compare the four kits, starting and elution volume must be the same
^dRNeasy Plus Micro Kit:maximum amount of starting material (animal and human cells): 5 × 10⁵, minimum elution volume: 14 μL

ISSN: 1471-2164